Association of SSCP Polymorphisms of HSP70 Gene with Physiological, Production and Reproduction Performance in Sahiwal and Crossbred Cows

Background: Cellular tolerance to heat stress is mediated by shock proteins (HSPs). The HSPs act as molecular chaperones and are transcribed in response stress. Among different families of these proteins, HSP70 considered be related the development temperature tolerance. Unraveling polymorphism protein genes could a step towards identification genetic markers for selecting heat-tolerant cattle. Methods: present study was carried out Sahiwal (n=50) Crossbred cows with objective identify polymorphisms gene. Two fragments (295 220 bp) gene were subjected Polymerase Chain Reaction-Single-Strand Conformation Polymorphism (PCR-SSCP) technique. Statistical analysis performed association each SSCP genotype on physiological, production reproduction traits crossbred using univariate GLM model SPSS 25. Result: PCR-SSCP 295 bp fragment revealed two genotypes AA AB AC cows. that had higher peak milk yield while same total lactation yield, lower service period calving interval found monomorphic both